Gene Expression in Specific Locations Questions
Gene Expression in Specific Locations Questions
Question Description
I’m working on a biology question and need guidance to help me learn.
1. (number 1-4) please provide a brief response.please state a hypothesis, your designated controls and experimental sample (tissue/embryonic stage/model organism as appropriate). State the results expected to support your hypothesis
a)Design an experiment that demonstrates the requirement of SRY in testis development. (6 sentences or less)
b)Design an experiment that would test if Sox9, a downstream target of SRY, is sufficient to compensate for the loss of SRY in testis development. (6 sentences or less)
Gene Expression in Specific Locations Questions
2. Enhancers are responsible for directing gene expression in specific locations and during specific times during development. For a gene that you know is essential for early development in the zebrafish, you have identified a 1000 bp sequence upstream that you suspect to be an enhancer.
a)Design an experiment and describe the result that would test when and where this enhancer would control gene expression.
B)Design an experiment that would test whether the 1000 bp sequence is sufficient to drive gene expression at a specific stage of development and/or in a specific tissue.
3. Design an experiment using beads soaked in a morphogen that can demonstrate that retinoic acid is sufficient for limb development.
a) What genes would you use as markers for fore- and hindlimb development?
b)State a marker that can be used to detect the posterior end of the developing limb.
c)How would you visualize the expression of these genes?
4. The AER (apical ectodermal ridge) is responsible for expressing morphogens such as FGF8 to maintain the growth of the developing limb bud.
a) Explain how FGF8 maintains a positive feedback loop during limb development and what tissues and signaling factors are involved.
b)Describe one experiment that demonstrates that FGF8 is sufficient to maintain limb growth.
5. In Hubaud et al. (2017), the authors observed that dissociated mouse PSM cells cultured on fibronectin do not exhibit oscillations. The authors restored oscillations in dissociated cells by (one to two sentences):
6.Briefly describe a congenital defect that arises from loss of the B-vitamin folic acid. What does this outcome tell you about nutrition?
7. Commissural neurons are found in the most dorsal region of the neural plate. Describe an experiment that shows that the floor plate expresses cues for neural migration, and identify that cue. Also describe what molecular changes occur in these cells that allow them to migrate to the brain at a 90 degree angle.
8.Design an experiment that addresses whether node cilia are, in part, responsible for L/R symmetry. State a gene you learned about in lecture that could be affected that would hinder the movement of cilia
9. Name two specific examples of an epithelial-to-mesenchymal transition that occurs during development (specific examples of tissues, not just the type of movement). Listing is fine:
10.Describe the guidance cue(s) that was/were found to be responsible for the movement of growth cones of retinal neurons to the tectum and how this guidance cue, along with its receptor, help retinal neurons conduct pathfinding. Include what part of the retina (N/T) maps to which part of the tectum (A/P).
11.Please explain what signaling pathway is responsible for determining webbed feet in ducks and the lack thereof in chickens.
12.Cells derived from the sclerotome make up the skeleton, bone and cartilage, while cells derived from the dermomyotome form muscle. Describe how sclerotome and dermomyotome are specified during development of the somite, and describe an experiment that demonstrates this process.
13.What oscillating signaling pathway induces somite segmentation in presomitic mesoderm? Word or phrase:
14.Snakes have rudimentary/vestigial structures in the ventral part of their bodies that demonstrate that at some point in time in their evolution, they lost the ability to form limbs. Please explain why snakes don’t have limbs.
15. a) There are three overarching types of experiments that are still used today that were derived from studies in developmental biology. State those three
b)Using the knowledge you have gained throughout this quarter, describe three experiments that exemplify these three types of experimental methods in the context of fertilization.
| Excellent | Good | Fair | Poor | ||
| Main Posting | 45 (45%) – 50 (50%)
Answers all parts of the discussion question(s) expectations with reflective critical analysis and synthesis of knowledge gained from the course readings for the module and current credible sources.
Supported by at least three current, credible sources.
Written clearly and concisely with no grammatical or spelling errors and fully adheres to current APA manual writing rules and style. |
40 (40%) – 44 (44%)
Responds to the discussion question(s) and is reflective with critical analysis and synthesis of knowledge gained from the course readings for the module.
At least 75% of post has exceptional depth and breadth.
Supported by at least three credible sources.
Written clearly and concisely with one or no grammatical or spelling errors and fully adheres to current APA manual writing rules and style. |
35 (35%) – 39 (39%)
Responds to some of the discussion question(s).
One or two criteria are not addressed or are superficially addressed.
Is somewhat lacking reflection and critical analysis and synthesis.
Somewhat represents knowledge gained from the course readings for the module.
Post is cited with two credible sources.
Written somewhat concisely; may contain more than two spelling or grammatical errors.
Contains some APA formatting errors. |
0 (0%) – 34 (34%)
Does not respond to the discussion question(s) adequately.
Lacks depth or superficially addresses criteria.
Lacks reflection and critical analysis and synthesis.
Does not represent knowledge gained from the course readings for the module.
Contains only one or no credible sources.
Not written clearly or concisely.
Contains more than two spelling or grammatical errors.
Does not adhere to current APA manual writing rules and style. |
|
| Main Post: Timeliness | 10 (10%) – 10 (10%)
Posts main post by day 3. |
0 (0%) – 0 (0%) | 0 (0%) – 0 (0%) | 0 (0%) – 0 (0%)
Does not post by day 3. |
|
| First Response | 17 (17%) – 18 (18%)
Response exhibits synthesis, critical thinking, and application to practice settings.
Responds fully to questions posed by faculty.
Provides clear, concise opinions and ideas that are supported by at least two scholarly sources.
Demonstrates synthesis and understanding of learning objectives.
Communication is professional and respectful to colleagues.
Responses to faculty questions are fully answered, if posed.
Response is effectively written in standard, edited English. |
15 (15%) – 16 (16%)
Response exhibits critical thinking and application to practice settings.
Communication is professional and respectful to colleagues.
Responses to faculty questions are answered, if posed.
Provides clear, concise opinions and ideas that are supported by two or more credible sources.
Response is effectively written in standard, edited English. |
13 (13%) – 14 (14%)
Response is on topic and may have some depth.
Responses posted in the discussion may lack effective professional communication.
Responses to faculty questions are somewhat answered, if posed.
Response may lack clear, concise opinions and ideas, and a few or no credible sources are cited. |
0 (0%) – 12 (12%)
Response may not be on topic and lacks depth.
Responses posted in the discussion lack effective professional communication.
Responses to faculty questions are missing.
No credible sources are cited. |
|
| Second Response | 16 (16%) – 17 (17%)
Response exhibits synthesis, critical thinking, and application to practice settings.
Responds fully to questions posed by faculty.
Provides clear, concise opinions and ideas that are supported by at least two scholarly sources.
Demonstrates synthesis and understanding of learning objectives.
Communication is professional and respectful to colleagues.
Responses to faculty questions are fully answered, if posed.
Response is effectively written in standard, edited English. |
14 (14%) – 15 (15%)
Response exhibits critical thinking and application to practice settings.
Communication is professional and respectful to colleagues.
Responses to faculty questions are answered, if posed.
Provides clear, concise opinions and ideas that are supported by two or more credible sources.
Response is effectively written in standard, edited English. |
12 (12%) – 13 (13%)
Response is on topic and may have some depth.
Responses posted in the discussion may lack effective professional communication.
Responses to faculty questions are somewhat answered, if posed.
Response may lack clear, concise opinions and ideas, and a few or no credible sources are cited. |
0 (0%) – 11 (11%)
Response may not be on topic and lacks depth.
Responses posted in the discussion lack effective professional communication.
Responses to faculty questions are missing.
No credible sources are cited. |
|
| Participation | 5 (5%) – 5 (5%)
Meets requirements for participation by posting on three different days. |
0 (0%) – 0 (0%) | 0 (0%) – 0 (0%) | 0 (0%) – 0 (0%)
Does not meet requirements for participation by posting on 3 different days. |
|
| Total Points: 100 | |||||
